عنوان مقاله [English]
To study the effects of ascorbate and temperature on antioxidant enzymes and germination indexes, a factorial experimental based on completely randomized block design with three replications was conducted at the Agricultural Research Laboratory of Mohaghegh Ardabili University in 2014. Treatments were three levels of ascorbate (control, 5 and 10 mM) and three levels of temperatures (15, 25 and 35 °C). Parameters under study were germination indices, production rates of antioxidant enzymes, proline and protein. The results of experiment indicated that ascorbate application that of raised proline content. Application of ascorbate resulted in enzymes reduction at 15°C more than 25°and 35° temperatures. Its application also reduced protein denaturation. Since sesame is a warm season plant, it seems that increasing antioxidant enzymes at 15°C is due to higher production of free radicals. The positive and significant correlation between antioxidant enzymes and germination indices represents their direct relationships. According to the results of this experiment, it seems that the application of ascorbate had positive effect on germination indices and reduced the effects of improper temperatures; it also benefited seedling growth of sesame.
Alivand, R., R, Tavakol Afshari, and F, SharifZadeh. 2012. Effect of Gibberellin, Salicylic acid and Ascorbic acid for improvement of germination qualification in Deteriorated seed of Oil-seed Rape. Journal of Iranian Crop Plant Science. 43(4): 561-571 (In Persian).
Anjum, N.A., S. Umar, and M.T. Chan. 2010. Ascorbate-Glutathione Pathway and Stress Tolerance in Plants. Springer Dordrecht Heidelberg London New York. 462p
Arora, A., R.K. Sairam, and G.C. Srivastava. 2002. Oxidative stress and antioxidative system in plants. Current science. 82(10): 1227-1238
Arrigo, A.P. 1998. Small stress proteins: chaperones that act as regulators of intracellular redox state and programmed cell death. Biological Chemistry. 379, 19–26.
Ashraf, M, and M.R. Foolad. 2007. Roles of glycinebetaine and proline in improving plant abiotic stress resistance. Environmental Experiment of Botany. 59: 206–216.
Basra, S.M., A.M, Farooq, and A. Khaliq. 2003. Comparative study of presowing seed enhancement treatments in indica rice (Oryza sativa L.), Pakistan Journal of Life and Societe Science. 1, 5-9.
Bates, L.S., R.P. Waldren, and I.D. Teare. 1973. Rapid determination of free proline for water stress studies. Plant soil. 39: 205-208.
Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annual Biochemistry. 72: 248-254.
Bradford, K.J., and H, Nonogaki. 2007. Seed Development, Dormancy and Germination. Blackwell Publishing Ltd. 389P.
Copeland, L.O, and M.B. McDonald. 1995. Principles of Seed Science and Technology. Pub. Chapman and Hall.USA. 488p.
Cosio, C., and Ch. Durand. 2009. Specific functions of individual class III peroxidase genes. Journal of Experimental Botany. 60:391–408
Damerval, C, D. Vienne, M. Zivy, and H. Thiellement. 1986. Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling proteins. Electrophoresis. 7: 52-54.
Demir Kaya, M., G. Okçu, M. Atak, Y. Çikili, and Ö. Kolsarici. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Europe Journal of Agronomy. 24, 291-295.
Diamant, S., N. Eliahu, D. Rosenthal, and P. Goloubinoff. 2001. Chemical chaperones regulate molecular chaperones in vitro and in cells under combined salt and heat stresses. Journal of Biological Chemistry. 276, 39586–39591.
Dolatabadian, A., S.A.M. Modarresi Sanavy, and K.S. Asilan. 2010. Effect of ascorbic acid foliar application on yield, yield component and several morphological traits of grain corn under water deficit stress conditions. Notulae Scientia Biologicae. 2: 45-50. (In Persian).
Finch-Savage, W.E., K.C, Dent, and L.J. Clark. 2004. Soak conditions and temperature following sowing influence the response of maize (Zea maize L.) seeds to on-farm priming (pre-sowing seed soak). Field Crop Research. 90, 361-374.
Foyer, C.H., and B. Halliwell. 1976. The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133:21–25
Gould, K., K. Davies, and Ch. Winefield. 2009, Anthocyanins (Biosynthesis, Functions, and Applications). Springer Science + Business Media, LLC. 345p
Green, B., M. Grevers, and G. Lafond. 1994. Soi1 temperature and crop emergence under conventional and direct seeding. Farm Facts. Canada-Saskatchewan Agreement on Soi1 Conservation. 400 pp.
Harris, D. 2003. Reducing risk and increasing yields from rain fed crops in Africa using on farm seed priming. 87-88. in Abstracts: Harnessing crop technologies to alleviate hunger and poverty in Africa. 6th Biennial Conference of the Africa Crop Science Society, Hilton Nairobi, Kenya. 12-16th October. pp: 87-88.
Izadi Darbandi, A., M. Mohammadian., A. Yang., H. Zarghani. 2012. Effect of temperature and salt on germination parameters and seed growth in Seaman (Sesamum indicum) population. Iranian Journal of Field Crops Research. 10(2): 335-345. (In Persian)
Job, C., L. Rajjou, Y. Lovigny., M. Belghazi, and D. Job. 2005. Patterns of protein oxidation in Arabidopsis seeds and during germination. Plant Physiology 138: 790–802.
Jordan, G.L., and M.R. Haferkamp. 1989. Temperature responses and calculated heat units for germination o f several range grasses and shrubs. Journal of Range Management. 42:41-45.
Kaffi, M, A. Nezami, H. Hosseini, and A. Masoumi. 2005. Physiological effect of drought stress due Poly etilen glycol on the lentil genotypes germination. Iranian journal of crop research. 81: 3-69. (In Persian)
Kar, M., and D. Mishra. 1976. Catalase, peroxidase and polyphenol oxidase activities during rice leaf senescence. Plant Physiology. 578: 315-319.
KaviKishor, P.B., S. Sangam, R. N. Amrutha, P. Sri Laxmi, K.R. Naidu, and K. Rao. 2005. Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: Its implications in plant growth and abiotic stress tolerance. Current Science.88(3): 424-438.
Kebreab, E., and A.J. Murdoch. 2000. The effect of water stress on the temperature range for germination of Orobanches aegyptiaca seeds. Seed Science Research. 10: 127-133.
Levitt, J. 1982. Stress terminology. In: N.C.Turner., P. J. Kramer (eds). Adaptation of plants to water and high temperature and stress. Wiley-Interscience, New York, PP: 7-20
Maestri, E., N. Klueva, C. Perrotta, M. Gulli, H.T. Hguyen, and N. Marmiroli. 2002. Molecular genetics of heat tolerance and heat shock proteins in cereals. Plant molcular. Biol. 48: 667-81.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science 7: 405-410
Mittler, R., S. Vanderauwera, M. Gollery, and F. Van Breusegem. 2004. Reactive oxygen gene network of plants. Trends Plant Science. 9(10): 490-498.
Naseri, F. 1991. Oil seeds. Publishing of Astan Quds Razavi cultural deputyship. 832Pp
Neubauer, C., and H.Y. Yamamoto. 1994. Membrane barriers and Mehler-peroxidase reaction limit the ascorbate availability for violaxanthin de-epoxidase activity in intact chloroplasts. PhotosyntheticResearch. 39:137–147
Noctor G, and CH., Foyer. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology Plant Molecular Biology 49:249–279
Nollen, E.A.A., and R.I. Morimoto. 2002. Chaperoning signaling pathways: molecular chaperones as stress-sensing ‘heat shock’ proteins. Journal of Cell Science 115:2809–2816.
Panchuk, I.I., R.A. Volkov, and F. Schoffl. 2002. Heat stress and heat shock transcription factor-dependent expression and activity of ascorbate peroxidase in Arabidopsis. Plant Physiology 129, 838–853.
Perry, D.A. 1980. The concept of seed vigor and its relevance to seed production techniques. Seed Production. Butter worth’s. London. 585-591.
Posmyk, M.M., and K.M. Janas. 2007. Effects of seed hydropriming in presence of exogenous proline on chilling injury limitation in Vignaradiata L. seedlings. Acta Physiology Plant
Quiroga, M., C. Guerrero, M.A. Botella, A. Barcelo, I. Amaya, M.I. Medina, F.J. Alonso, S.M. De Forchetti, H. Tigier, and V. Valpuesta. 2000. A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiology. 122: 1119-1127.
Rabie, B., and M. Bayat. 2009. Study parameters of seed germination and seedling growth canola cultivars (Brassica napus L.) by using seed vigor tests. Iranian Journal of Crop Sciences. 40(1):93-104. (In Persian)
Scott, S.J., R.A. Jones, and W.A. Williams, 1984. Review of data analysis methods for seed germination. Crop Science. 24: 1192-1199.
Singh Gill, S., and N. Tuteja, 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants: A Review. Plant Physiology and Biochemistry. 48: 909-930.
Soltani, A., M. Gholipoor, and E. Zeinali. 2006. Seed reserve utilization and seedling of wheat as affected by drought and salinity. Environmental and Experimental Botany. 55: 195-200
Stasolla, C., and E.C. Yeung, 2001. Ascorbic acid metabolism during white spruce somatic embryo maturation and germination. Physiology Plant. 111:196–205
Sudhakar, C., A. Lakshmi, and K.S. Giridara, 2001. Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity. Plant Science. 167:613-619
Tozlu, I., G.A. Moore, and C.L Guy. 2000. Effect of increasing NaCl concentration on stem elongation, dry mass production and macro –nutrient accumulation in Poncirus trifoliata. Australian Journal of Plant Physiology 27: 35-42.
Turkan, I. 2011. Plant responses to drought and salinity stress, Development in a post-Genomic era. Advances in Botanical Research. 593p.
Weise, E.A. 2000. Oil seed crops. Blackwell Sci. Ltd Oxford, UK. 364 P.
Zand, B., A. Soroushzadeh, F. Ghanati, and F. Moradi. 2010. Effect of foliar application of zinc (Zn) and auxin (IBA) on the activity of some antioxidant enzymes in the corn. Iran Journal o Plant Biology. 2:48-35.(In Persian)