تغییرات صفات مورفولوژیک و فیزیولوژیک شاهدانه ((Cannabis sativa L. متاثر از پرایمینگ بذر با فولیک اسید و پراکسید هیدروژن

نوع مقاله: علمی پژوهشی

نویسندگان

1 گروه زراعت و اصلاح نباتات، واحد مهاباد، دانشگاه آزاد اسلامی، مهاباد، ایران

2 استادیار گروه کشاورزی، دانشگاه پیام نور، سقز، ایران

چکیده

به منظور بررسی اثر پرایمینگ بذور شاهدانه با فولیک اسید و پراکسید هیدروژن بر برخی صفات مورفولوژیک و فیزیولوژیک، آزمایشی در گلخانه دانشگاه آزاد اسلامی واحد مهاباد اجرا گردید. فاکتورهای آزمایشی شامل پراکسید هیدروژن در پنج سطح (صفر، 5/7، 15، 5/22، 30 میلی­مول در لیتر) و فاکتور دوم فولیک اسید در پنج سطح (صفر، 5، 10، 20، 27 میلی­مول در لیتر) بودند که به صورت فاکتوریل در قالب طرح کاملاً تصادفی در چهار تکرار اجرا شد. به منظور پیش­تیمار کردن بذرها پس از تهیه محلول­های مورد نظر، بذر شاهدانه به مدت 24 ساعت در اسید فولیک و 6 ساعت در پراکسید هیدروژن به­صورت غوطه­ور قرار گرفتند. در این مطالعه صفات کلروفیل a، b، کلروفیل کل، محتوی نسبی آب، ارتفاع بوته، طول ریشه، ضریب آلومتری، وزن تر و خشک بوته اندازه­گیری شدند. نتایج تجزیه واریانس داده­ها نشان داد که اثر پراکسید هیدروژن و اسید فولیک بر کلیه صفات مورد بررسی معنی­دار بود، اما اثر متقابل بین دو تیمار تنها بر صفات محتوی نسبی آب و ضریب آلومتری معنی­دار گردید. پرایمینگ بذر با سطح 15 میلی­مول در لیتر پراکسید هیدروژن و 5 میلی­مول در لیتر اسید فولیک بالاترین مقدار کلروفیل a، کلروفیل b، کلروفیل کل، ارتفاع ساقه، طول ریشه و وزن تر و خشک بوته را به خود اختصاص دادند. افزایش سطح پراکسید هیدروژن بیشتر از 15 میلی­مول در لیتر، اثر منفی بر کلیه صفات مورد مطالعه نشان داد. همچنین، ترکیب تیماری 15 میلی­مول در لیتر پراکسید هیدروژن و 5 و10 میلی­مول اسید فولیک در مقایسه با دیگر تیمارها بالاترین محتوی نسبی آب و ضریب آلومتری را به خود اختصاص دادند. بنابراین، با توجه به نتایج مطالعه حاضر، پرایمینگ بذر با سطح 15 میلی­مول در لیتر پراکسید هیدروژن و 5 میلی­مول در لیتر اسید فولیک جهت حصول صفات مطلوب مورفولوژیک و فیزیولوژیک شاهدانه در شرایط این آزمایش مناسب می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Morpho- Physiological Changes of Hempseed (Cannabis sativa L.) Traits as Affected by Seed Priming with Folic Acid and Hydrogen Peroxide

نویسندگان [English]

  • Shirin Karbalaye Golizadeh 1
  • Touraj Mir Mahmoodi 1
  • Nabi Khalili Aqdam 2
1 Department of Agronomy and Plant Breeding, Mahabad Branch,Islamic Azad University, Mahabad,Iran
2 Department of Agriculture, Payam noor University of Bokan, Bokan, Iran
چکیده [English]

To evaluate the effects of seed priming of hempseed with folic acid and hydrogen peroxide on some morphological and physiological traits a factorial greenhouse experiment based on randomized complete design with four replications was conducted at Islamic Azad University, Mahabad Branch. Treatments consisted of hydrogen peroxide at five levels (0, 7.5, 15, 22.5, 30 mm/liter) as the first factor and the four leveld of folic acid (5, 10, 20, 27 mm/liter) as the second factor.Seeds, to be primed, were immersed into solution of folic acid for 24 hours and hydrogen peroxide for 6 hours. The characteristics like chlorophyll a, chlorophyll b, total chlorophyll content, relative water content, plant height, root length, allometric coefficient, plant fresh and dry weights, were measured. Result of analysis of variance showed that the effects of folic acid and hydrogen peroxide on all characters were significant, but the interaction between the two treatments were only significant on relative water content and allometric coefficient. In this study, seed priming with 15 mm/liter of hydrogen peroxide and 5mm folic acid resulted in highest chlorophyll a, chlorophyll b, total chlorophyll contents, plant height, root length, fresh and dry weights. Increasing hydrogen peroxide level above 15mm/liter affected traits negatively. Combinated treatments of 15 mm/liter of hydrogen peroxide and 5 and 10 mm of folic acid resulted in highest relative water content and allometric coefficient, respectively. Based on the results obtained it can be concluded that priming seeds with 15 mm/liter of hydrogen peroxide and 5mm folic acid is recommended to produce proper morphological and physiological traits.

کلیدواژه‌ها [English]

  • Cannabis
  • folic acid
  • hydrogen peroxide
  • seed priming
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