کاهش تنش ناشی از سمیت آرسنیک در سویا (Glycine max L) با استفاده از سدیم نیتروپروساید

نوع مقاله: علمی پژوهشی

نویسندگان

1 گروه زیست شناسی، دانشکده علوم، دانشگاه شهید باهنر کرمان، کرمان، ایران

2 پژوهشکده علوم محیطی، مرکز بین المللی علوم و تکنولوژی پیشرفته و علوم محیطی کرمان، ایران

چکیده

آرسنیک یکی از مهم ­ترین سموم آلوده­ کننده­ی محیط زیست محسوب می ­شود. آرسنیک به طرق مختلف از جمله تشکیل گونه­ های اکسیژن فعـال و در نتیجه پراکسیداسیون لیپیدهای غشـایی باعث اختلال در رشــد گیاهان می­ شود. در این مطالعه اثر متقابل آرسنیک و سدیم نیترو پروساید (SNP) بر گیاه سویا بررسی شد. بدین منظور، سویا در مرحله چهار برگی مورد تیمار غلظت­ های مختلف آرسنیک (0 ،150 و300 میکرومولار) و SNP (صفر و 100میکرومولار) قرار گرفت و صفاتی نظیر غلظت پراکسید هیدروژن، پرولین، مالون دی­آلدهید و فعالیت برخی آنزیم ­های آنتی اکسیدان در شاخساره اندازه­ گیری شد. نتایج نشان داد که با افزایش غلظت آرسنیک در محیط هوگلنـد، میزان کلروفیل کل در شاخساره بـه­ طـور معنی­ داری کاهش و فعالیت آنزیم ­های کاتالاز و پراکسیداز به­ طور معنی­ داری افزایش یافت. همچنین، در تیمارهای آرسنیک تجمع پراکسید­هیدروژن به­ طور معنی­ داری افزایش یافت که منجر به پراکسیداسیون لیپیدهای غشایی گردید. افزایش محتوای مالون ­دی­آلدهید، این را تأیید نمود. به کارگیری SNP در محـیط حـاوی آرسنیک، میزان کلروفیل کل، فعالیت آنزیم ­های آسکوربات پراکسیداز و گایاکول پراکسیداز را به ­طور معنی­داری افزایش داد ولی، فعالیت آنزیم کاتالاز با اسـتفاده از SNP در این شرایط کاهش پیدا کرد. بنـابراین، SNP در کـاهش آسـیب­ هـای اکسایشی می ­تواند نقش مؤثری داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Reducing Arsenic Toxicity Stress in Soybean (Glycine max L.) by Using of Sodium Nitroprusside

نویسندگان [English]

  • Elham Asadi karam 1
  • Batool Keramat 1
  • Hossein Mozaffari 2
1 Department of Biology, Faculty of Science, Shahid Bahonar University, Kerman, Iran
2 Environmental Research Institute, the International Center for Advanced Science and Technology, and Environmental Sciences Kerman, Iran
چکیده [English]

Arsenic contamination is one of the most important compounds all over the world. Arsenic in different ways, including the formation of reactive oxygen species and membrane lipid peroxidation impairs growth of plants. In this study, the effect of arsenic and sodium nitroprusside (SNP) were evaluated on soybean. Soybean at four leaf stage was treated with different concentrations of arsenic (0, 150 and 300 mM) and SNP (0 and 100 mM), and then concentrations of hydrogen peroxide, proline, malondialdehyde and activity of antioxidant enzymes in its shoot were measured. The results showed that increasing concentration of arsenic in Hoagland solution reduced total chlorophyll content in the shoot and increased the activity of catalase and peroxidase significantly. It was also observed that treating plants with arsenic increased hydrogen peroxid accumulation which resulted in peroxidation of membrane lipids. Higher malondialdehyde content confirmed this result. Using SNP in the medium containing arsenic increased total chlorophyll content, activities of guaiacol peroxidase enzyme and ascorbate peroxidase, significantly. However, the catalase activity in this case decreased. Thus it can be concluded that using SNP, would reduce effectively the damage of oxidation.

کلیدواژه‌ها [English]

  • Arsenic
  • antioxidants
  • Sodium nitroprusside
  • soybean

Allen, R. 1995. Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology. 107: 1049- 1054.

Arasimowicz-Jelonek, M., and J. Floryszak-Wieczorek. 2007. Nitric oxide as a bioactive signalling molecule in plant stress responses. Plant Science. 172: 876-887

Arasimowicz-Jelonek, M., J. Floryszak-Wieczorek, and J. Kubis. 2009. Involvement of nitric oxide in water stress-induced responses of cucumber roots. Plant Science. 177: 682-690.

Bates, L.S. 1973. Rapid determination of free proline for water stress studies. Plant and Soil. 39: 205-207.

Behnamnia, M., K. Manouchehri Kalantari, and J. Ziaie. 2009. Effects of brassinosteroid on the induction of biochemical changes in Lycopersicon esculentum under drought stress. Turkish Journal of Botany. 33: 417-428.

Beligni, M.V., and L. Lamattina. 1999. Nitric oxide counteracts cytotoxic processes mediated by reactive oxygen species in plant tissues. Planta. 208: 337-344

Bor, M., F. Özdemir, and I. TüKan. 2003. The effect of salt stress on lipid peroxidation and antioxidants in leave of sugar beet (Beta vulgaris L.) and wild beet (Beta maritime L.). Journal of Plant Science. 163: 77-84

Bradford, M.M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 72: 248-254.

Chun-xi, L., F. Shu-li, S.H. Yan, J. Li-na, L. Xu-yang, and H. Xiao-li. 2007. Effects of arsenic on seed germination and physiological activities of wheat seedlings. Journal of Environmental Science. 19: 725-732.

Connell, S.L., and S.H. AL-Hamdani. 2001. Selected physiological responses of Kudzu to different chromium concentration. Canadian Journal of Plant Science. 81: 33-58.

Del Rio, L.A., F.J. Corpas, and J. B. Barroso. 2004. Nitric oxide synthase activity in plants. Phytochemistry. 65: 783- 792.

Deo, B., and P.K. Nayak. 2011. Study of copper phytotoxicity on in vitro culture of Musa acuminata cv.‘Bantala’. Journal of Agricultural Biotechnology Sustainable Development. 3(8): 136-140.

Dhindsa, R.S., P. Plumb–Dhindsa, and T.A. Thrope. 1981. Leaf senescence: correlated with increased levels of membrane permeability and lipid per oxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany. 32: 43-101.

Duan, X., X. Su, Y. You, H. Qu, Y. Li, and Y. Jiang. 2007. Effect of nitric oxide on pericarp browing of harvested longan fruit in relation to phenolic metabolism. Food Chemistry. 104: 571-576.

Duquesnoy, I., G.M. Champeau, G. Evray, G. Ledoigt, and A. Piquet-Pissaloux. 2010. Enzymatic adaptations to arsenic-induced oxidative stress in Zea mays and genotoxic effect of arsenic in root tips of Vicia faba and Zea mays. Comptes Rendus Biologies. 333: 814-824

Gunes, A., D.J. Pilbeam, and A. Inal. 2009. Effect of arsenic- phosphorus interaction on arsenic-induced oxidative stress in chickpea plants. Plant and Soil. 314: 211-220

Hall, J.L. 2002. Cellular mechanism for heavy metal detoxification and tolerance. Journal of Experimental Botany. 53(336): 1 -11.

Heath, R. L., and L. Packer. 1969. Photo peroxidation in isolated chloroplast: kinetic and stoichiometry of fatty acid peroxidation. Archive of Biochemistry and Biophysics. 125: 189-198.

Herbinger, K., M. Tausz, A. Wonisch, G. Soja, A. Sorger, and D. Grill. 2002. Complex interactive effects of drought and ozone stress on the antioxidant defense systems of two wheat cultivars. Plant Physiology and Biochemistry. 40: 691-696.

Jain, M., and R. Gadre, 2004. Inhibition of 5-amino levulinic acid dehydratase activity by arsenic in excised etiolated maize leaf segments during greening. Plant Physiology. 161: 251-255

Jasid, S.N., M. Simontacchi, and S. Puntarulo. 2008. Exposure to nitric oxide protects against oxidative damage but increases the labile iron pool in sorghum embryonic axes. Journal of Experimental Botany. 12: 1-10.

Jha, A.B., and R.S. Dubey. 2004. Carbohydrate metabolism in growing rice seedling under arsenic toxicity. Plant Physiology. 161: 867-872.

Karimi, N., S.M. Ghaderian, H. Marofi, and H. Schat. 2010. Analysis of arsenic in soil and vegetation of a contaminated area in Zarshuran, Iran, identify two angiosperm arsenic hyperaccumulators. International Journal Phytoremediation. 12: 159-173.

Kranner, I., and L. Colville. 2011. Metals and seeds: Biochemical and molecular implication and their significance for seed germination. Journal of Experimental Botany. 72: 93-105.

Lei, Y., C. Yin, J. Ren, and C. Li. 2007. Effect of osmotic stress and sodium nitroprusside pretreatment on proline metabolism of wheat seedlings. Biologia Plantarum. 51: 386-390.

Lester, G.E., and F. Stein. 1993. Plasma membrane physicochemical changes during maturation and postharvest storage of muskmelon fruit. Journal of Horticultural Science and Biotechnology. 118: 223-227.

Lichtenthaler, H.K. 1987. Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. Method Enzymol. 148: 350-382

Liu, Q., C. Hu, Q. Tan, X. Sun, J. Su, and Y. Liang. 2008. Effects of as on as uptake, speciation and nutrient by winter wheat (Triticum aestivum) under arsenate co contamination. Ecotoxicology Environmental Safety. 68: 505-313.

Meharg, A.A., and M.R. Macnair. 1992. Genetic correlation between arsenate tolerance and the rate of arsenate and phosphate uptake in Holcus lanatus L. Heredity. 69: 336-341.

Miteva, E., and M. Merakchysk. 2002. Response of chloroplasts and photosynthetic mechanism of bean plants to excess arsenic in soil. Bulgarian Agricultural Science. 8: 151-156

Mithofer, A., B. Schulze, and W. Boland. 2004. Biotic and heavy metal stress response in plants: evidence for common signals. FEBS Letters. 566: 1-5.

Morel, F.M.M. 2008. The co-evolution of phytoplankton and trace element cycle in the oceans. Geobiology. 6: 318-24.

Nakano, Y., and K. Asado. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiology. 22(5): 867-880.

Nasibi, F., M.M. Yaghoobi, and Kh. Kalantari. 2011. Effect of exogenous arginine on alleviation of oxidative damage in tomato plant under water stress. Plant Interactions. 6: 291-296.

Neill, J., D. Radhika, and J. Hancock. 2003. Nitric oxide signaling in plant. New Phytologists. 159: 11-35.

Panda, P., Sh. Nath, Th.Th. Chanu, G.D. Sharma, and S.K. Panda 2011. Cadmium stress-induced oxidative stress and role of nitric oxide in rice (Oryza sativa L.). Acta Physiologiae Plantarum. 33: 1737-1747

Plewa, M.J., S.R. Smith, and E.D. Wanger. 1991. Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation Research. 247: 57-64.

Roe, J.H. 1955. The determination of sugar in blood and spinal fluid with anthrone reagent. Journal of Biological Chemistry. 212: 335-343.

Roy, P., and A. Saha. 2002. Metabolism and toxicity of arsenic: A human carcinogen. Current Science. 82: 38-45.

Shaibur, M.R., N. Kitajima, R. Sugawara, T. Kondo, Sh. Alam, S.M. Imamul-Huq, and Sh. Kawai. 2008. Critical toxicity level of arsenic and elemental composition of arsenic-Induced chlorosis in hydroponic sorghum. Water Air and Soil Pollution. 191: 279-292.

Sheokand, S., A. Kumari, and V. Sawhney. 2008. Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chick pea plants. Physiology and Molecular Biology of Plants. 14(4): 355-362.

Shri, M., S. Kumar, D. Chakrabarty, P.K. Trivedi, S. Mallick, P. Misra, D. Shukla, S. Mishra, S. Srivastava, R.D. Tripathi, and R. Tuli. 2009. Effect of arsenic on growth, oxidative stress, and antioxidant system in rice seedlings. Ecotoxicology and Environmental Safety. 72: 1102-1110.

Singh, H.P., D.R. Batish, R.K. Kohli, and K. Arora. 2007. Arsenic induced root growth inhibition in mung bean (Phaseolus aureus Roxb) is due to oxidative stress resulting from enhanced lipid peroxidation. Plant Growth Regulation. 5: 65-73.

Singh, H.P., S. Kaur, D.R. Batish, V.P. Sharma, and N. Sharma. 2009. Nitric oxide alleviates arsenic toxicity by reducing oxidative damage in the roots of Oryza sativa (rice). Nitric Oxide. 20: 289-297.

Stancheva, I., N. Kaloianova, and E. Atanasova. 1999. Effect of copper and arsenic on the yield and plastid pigment content of rice inoculated with Azospirilum Brasilense. Soil Science. 39(4-5): 140-143.

Stoeva, N., and T.Z. Bineva. 2003. Oxidative changes and photosynthesis in Oat plants grow in as contaminated soil. Plant Physiology. 29: 87-95

Stoeva, N., M. Berova, and Z. Zlatez. 2004. Physiological response of maize to arsenic contamination. Planta. 47(3): 449-452.

Sun, B., J. Yan, K. Chen, L. Song, F. Chen, and L. Zhang. 2007. Protective effect of nitric oxide on iron deficiency-induced oxidative stress in maize (Zea mays). Plant Physiology. 164: 536-543.

Tu, C., and L. Q. Ma. 2003. Effects of arsenate and phosphate on their accumulation by an arsenic hyperaccumulator Pteris vittata L. Plant and Soil. 249: 373-382.

Uchida, A., T. Jagendorf, and T. Hibino. 2002. Effects of hydrogen peroxide and nitric oxide on both salt and heat stress tolerance in rice. Plant Science. 163: 515-523.

Vazquez, S., E. Esteban, and R.O. Carpena. 2008. Evolution of arsenate toxicity in nodulated white lupine in a long-term culture. Journal of Agricultural and Food Science. 56(18): 8580-8587.

Velikova, V., I. Yordanov, and A. Edreva. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science. 151: 59-66.

Verbruggen, N., and C. Hermans. 2008. Proline-accumulation in plants: a review. Amino Acids. 35(4): 753-759.

Wang, X., S. Guoxin, X. Qinsong, and H. Jinzhao. 2006. Exogenous polyamines enhance copper tolerance of Nymphoides peltatum. Plant Physiology. 164: 1062-1070.

Wieczorek, J.F., G. Milczarek, M. Arasimovicz, and A. Ciszewski. 2006. Do nitric oxide donors mimic endogenous NO-related response in plants. Planta. 224: 1363-1372.

Yu, T.Q., L.N. Chai, and Z.P. Liu. 1995. Expression of the soluble protein in water-stressed wheat seedlings and the drought-resistant proteins. Beijing Agricultural College. 10(1): 26-31

Zengin, F.K., and O. Munzuroglu. 2005. Effects of some heavy metals on content of chlorophyll, proline and some antioxidant chemicals in bean (Phaseolus vulgaris L.) seedlings. Acta Biological Cracoviensia Series Botanica. 47: 157-164.

Zhang, Z., O. Pang, X. Duan, Z.L. Ji, and Y. Jiang. 2005. Role of peroxidase in anthocyanine degradation in litchi fruit pericarp. Food Chemistry. 90: 47-52

Zhao, F.J., J.F. Ma, A.A. Meharg, and S.P. Mc Grath. 2009. Arsenic uptake and metabolism in plants. New Phytologist. 181: 777-794.

Zhu, S., M. Liu, and J. Zhou. 2006. Inhibition by nitric oxide of ethylene biosynthesis and lipoxygenase activity in peach fruit during storage. Postharvest Biology and Technology. 42: 41 -48.