اثر سالیسیلیک اسید و اتفن بر جوانه زنی بذر و رشد دانه‌رست گندم تحت تنش شوری

نوع مقاله: علمی پژوهشی

نویسندگان

1 کارشناسی ارشد گروه زیست شناسی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران

2 استادیار گروه زیست شناسی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران

چکیده

شوری آب یا خاک از مهم ­ترین عواملی است که باعث کاهش جوانه‌زنی بذور گیاهان می‌شود. اتفن می‌تواند خواب بذر را در انواعی از گیاهان مثل غلات بشکند و جوانه‌زنی را سرعت بخشد. در برخی گیاهان پیش تیمار بذرها با سالیسیلیک اسید سبب افزایش درصد جوانه ­زنی شده است. به­ منظور بررسی اثر سالیسیلیک اسید و اتفن بر جوانه‌زنی بذور و رشد دانه‌رست رقم سیوند گندم تحت تنش شوری، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در بهار سال 1390 و در آزمایشگاه تحقیقات گیاهی دانشگاه آزاد اسلامی واحد نیشابور انجام شد. شوری با سطوح صفر، 50، 100و150 میلی مولار NaCl، سالیسیلیک اسید در سطوح صفر، 0.5 و 1 میلی مولار و اتفن در سطوح صفر، 0.05، 0.1 و 0.2 میلی مولار استفاده شد. نتایج نشان داد که درصد و سرعت جوانه‌زنی، طول و وزن خشک اندام هوایی و ریشه‌ی ‌اولیه و میزان فعالیت آنزیم آلفا آمیلاز در شرایط شوری کاهش و میزان پرولین افزایش یافت و پیش‌تیمار بذور با سالیسیلیک اسید سبب افزایش درصد جوانه‌زنی، برخی صفات رشدی و میزان فعالیت آنزیم آلفا آمیلاز و میزان پرولین در محیط شور گردید. همچنین، پیش‌تیمار بذور با اتفن در شرایط شور باعث کاهش برخی صفات رشدی و افزایش میزان پرولین شد ولی بر صفات جوانه‌زنی و میزان فعالیت آنزیم آلفا آمیلاز بی‌تاثیر بود. به نظر می­ رسد سالیسیلیک اسید به عنوان یک تنظیم کننده رشد گیاهی و مؤثر در شرایط تنش شوری و اتفن از طریق تبدیل شدن به اتیلن، مکانیسم‌های تحمل گیاه را فعال و باعث بهبود خسارات ناشی از شوری بر جوانه‌زنی و رشد دانه‌رست گندم شده‌اند.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Salicylic Acid and Ethephon on Seed Germination and Seedling Growth of Wheat under Salt Stress

نویسندگان [English]

  • Soheyla Shakeri 1
  • Fatemeh Saeid Nematpour 2
  • Akbar Safipour Afshar 2
1 Graduate Student, Department of Biology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2 Assistant professor, Department of Biology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
چکیده [English]

Water or soil salinities are the most important factors that reduce the seed germination of plants. Ethephon can break seed dormancy in a variety of plants, such as cereals and speeds up germination. In some plants pretreatment of seeds with salicylic acid has increased the germination percentage. To study effect of salicylic acid and ethephon on seed germination of wheat (Seivand cultivar) under salinity condition a factorial experiment in a completely randomized design with three replications was conducted at the Plant Research Laboratory of Neyshabur Branch of Islamic Azad University in 2011. Four salinity levels (0, 50, 100, 150 mM), three salicylic acid levels (0, 0.5, 1 mM) and four ethephon levels (0, 0.5, 1, 2 mM) were used. The results showed that at salinity condition seed germination rate and percentage, shoot and root length, their dry weight and α-amylase activity decreased and proline content increased. Pretreatment of seeds by salicylic acid increased seed germination percentage, some growth parameters, α-amylase activity and proline content under salinity condition. Moreover, pretreatment of seeds by ethephon decreased some growth parameters and increased proline content but its effect on germination and α-amylase activity were not significant. It seems that Salicylic acid as a plant growth regulator under salinity condition and ethephon convertion to ethylene, activated plant tolerance mechanisms to salinity condition and decrease damaging effect of salinity on seed germination and seedling growth of wheat.

کلیدواژه‌ها [English]

  • α-amylase
  • cereals
  • ethylene
  • germination
  • Proline
  • Salinity

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