ارزیابی لاین های اینبرد نوترکیب عدس با استفاده از شاخص های تحمل به خشکی

نوع مقاله: علمی پژوهشی

نویسندگان

1 دانشگاه شهرکرد، دانشکده کشاورزی، گروه اصلاح نباتات و بیوتکنولوژی

2 استاد گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد، ایران

چکیده

به‌منظور تحمل به خشکی 168 لاین اینبرد نوترکیب عدس حاصل از تلاقی رقم هندی L3685 (پر محصول، با عادت رشدی ایستاده و زودرس) به‌عنوان والد مادری و رقم ایرانی قزوین (با عادت رشدی خوابیده و دیررس) به‌عنوان والد پدری، آزمایشی تحت دو شرایط بدون تنش و تنش خشکی پایان دوره رشد در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در مزرعه تحقیقاتی دانشگاه شهرکرد در سال زراعی 92-1391 به اجرا درآمد. نتایج تجزیه واریانس مرکب داده­ ها در شرایط فوق نشان داد که بین شرایط و همچنین بین لاین‌های مورد بررسی از نظر عملکرد دانه اختلاف معنی‌دار (P≤0.01) وجود دارند. معنی‌دار بودن اثر متقابل لاین × شرایط، بیانگر وجود اختلاف در لاین‌ها از نظر عملکرد دانه نسبت به تنش خشکی پایان دوره بود. لاین‌های 160، 125 و 129 در هر دو شرایط به‌عنوان متحمل­ ترین لاین‌ها به خشکی از لحاظ عملکرد دانه شناخته شدند. همبستگی مثبت و معنی‌دار بین شاخص‌های تحمل و عملکرد دانه در شرایط تنش و عدم تنش، بیـانگر برتـری شـاخص‌های STI، MP، HARM و GMP در شنـاسایی لاین‌های متحمل و حساس می ­باشند. بر اساس شاخص‌های فوق لاین‌های 160، 125، 48، 103 و 129 به‌عنوان لاین‌های متحمل شناخته شدند. با توجه به نتایج تجزیه به مؤلفه‌های اصلی، مؤلفه اول و دوم در مجموع 97/8 درصد از تنوع موجود در میان لاین‌ها برای شاخص‌های مقاومت به خشکی را توجیه نمودند. توزیع لاین‌ها در فضای بای پلات وجود تنوع ژنتیکی بین آنها نسبت به تنش خشکی را نشان داد و بر این اساس لاین‌های 160، 125، 48، 129 و 103  به‌عنوان لاین‌های متحمل و با عملکرد بالا شناسایی شدند.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of Lentil Recombinant Inbred Lines using Drought Tolerance Indices

نویسندگان [English]

  • Mohammad Hassan Rahimi 1
  • Sadollah Houshmand 2
  • Mahmood Khodambashi 2
1 Ph.D. Student of Plant Breeding, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran
2 Prof., Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Shahrekord, Shahrekord, Iran
چکیده [English]

To identify drought tolerant lines among 168 recombinant inbred lines derived from a cross between Hindi variety of L3685 (high yielding, erect type and early growth) as female parent and Iranian variety of Qazvin (with prostrate growth and late growth) as male parent, two experiments were conducted in randomized complete block design with three replications under non-stress and drought stress conditions at the experimental Field of Shahrekord University in 2013. Results of combined analysis of variance showed that there were significant differences among lines under study in both experimental conditions in terms of grain yield (P≤0.01). Significant interaction of line×conditions revealed that lines responded differently to drought stress based on grain yield. Results also indicates that lines 160, 125 and 129 were promising lines under both conditions in terms of grain yield. Positive and significant correlation between tolerance indices and grain yield under stress and non-stress conditions indicated that the STI, MP, HARM and GMP indices may identify promising from non promising lines. Based on the above criteria, lines 160, 125, 48, 103 and 129 were recognized as drought tolerant lines. According to the results of principal components analysis, the first and second components determined 97.8% of the total variations among genotypes under study for drought resistance indices. The distribution of lines in the biplot showed genetic variations to the drought stress and thus lines 160, 125, 48, 129 and 103 were identified as high yielders under drought stress condition.

کلیدواژه‌ها [English]

  • Correlation
  • Drought tolerance Indices
  • Grain yield
  • Lentil
  • Recombinant inbred line

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