بررسی کمیت و کیفیت علوفه شبدر برسیم ‏‎(Trifolium alexandrinum L.) ‎‏ ‏با کاربرد سویه‌‌های باکتری ‏Pseudomonas putida‏ و کود فسفره در کشت دوم

نوع مقاله: علمی پژوهشی

نویسندگان

1 استادیار گروه زراعت دانشگاه آزاد اسلامی واحد رشت

2 دانش‌آموخته کارشناسی ارشد زراعت، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران. ‏

چکیده

اثر برخی از سویه‌های باکتری سودوموناس پوتیدا همراه با سطوح مختلف کود شیمیایی فسفر بر خصوصیات کمّی و کیفی علوفه شبدر برسیم طی آزمایشی به­صورت فاکتوریل با طرح بلوک‌های کامل تصادفی در سه تکرار در منطقه فومن استان گیلان به­صورت کشت دوم، پس از برداشت برنج، بررسی شد. سطوح کودی شامل سه سطح فسفر (صفر، ۷۵ و 1۵0 کیلوگرم سوپرفسفات تریپل در هکتار) و سویه‌های باکتری در چهار سطح (سویه‌های M21،  M168و M5 و بدون تلقیح با باکتری) بودند. نتایج نشان داد که کاربرد کود فسفر منجر به افزایش pH خاک در طول دوره رشد گیاه شد، در حالی که سویه‌های باکتری pH خاک را تعدیل نمودند. همچنین، تلقیح باکتریایی مقدار پروتئین خام، پروتئین قابل هضم علوفه، فعالیت فسفاتاز اسیدی و قلیایی خاک پیرامون ریشه را در مقایسه با تیمار عدم تلقیح افزایش ولی مقدار الیاف خام علوفه را کاهش داد. عملکرد علوفه تر شبدر، عملکرد پروتئین و مقدار فسفر شاخ و برگ نیز تحت تاثیر برهمکنش سویه‌های باکتری و کود شمیایی فسفر قرار گرفتند. بیشترین عملکردهای علوفه تر و پروتئین به­ترتیب با میانگین­های 16۱36 و ۲۷۴۶ کیلوگرم در هکتار از سویه M5 در سطح ۱۵۰ کیلوگرم کود فسفات به­دست آمدند که نسبت به تیمار عدم تلقیح، عملکردهای علوفه و پروتئین را به­ترتیب ۴۹/۱۶ و ۱/۸ درصد افزایش داد. با توجه به نتایج به­دست آمده، کاربرد ۱۵۰ کیلوگرم کود شیمیایی فسفر در هکتار و تلقیح شبدر با باکتری سویه M5 سودوموناس پوتیدا برای دست­یابی به حداکثر عملکرد و کیفیت علوفه آن در نظام کشت دوم در منطقه فومن می­تواند مؤثر باشد.  

کلیدواژه‌ها


عنوان مقاله [English]

Forage Quantity and Quality of Berseem Clover (Trifolium ‎alexandrinum L.) as Affected by Uses of Pseudomonas putida ‎Strains and Phophorus Fertilizer in the Second Crop

نویسندگان [English]

  • Mohammad Hossein Ansari 1
  • Mehdi Ghadimi 2
1 Assistant Professor, Department of Agronomy and Plant Breeding, Rasht Branch, Islamic Azad University, Rasht, Iran
2 M.Sc. Department of Agronomy and Plant Breeding, Rasht Branch, Islamic Azad University, Rasht, Iran.‎
چکیده [English]

Effects of phosphate fertilizer and pseudomonas putida strains on the quantity and quality of forage of berseem clover as a second crop was studied in a factorial field experiment using randomized complete block design with three replications at Fooman, Guilan province, Iran. Treatments consisted of phosphate fertilizer with three levels (0, 75 and 150 kg/ha as triple super phosphate) and Pseudomonas putida strains with four levels (M21, M5, M168 and control). The results showed that use of phosphate fertilizers increased the soil pH during growing season while bacterial inoculation adjusted soil pH. The bacterial inoculation increased amount of crude protein, digestible protein, acidic and alkaline phosphatase activity compared to non-inoculated treatment, but it decreased crude fiber of the forage. Clover forage yield, protein yield and phosphorus content of foliage also were influenced by the interaction of bacterial strains and phosphate fertilizer. The highest forage and protein yield were obtained by using strain M5+150 kg P ha-1. Significant increases in forage and protein yield were found to be 16.49% and 8.01%, respectively, as compared with non-inoculated treatment. Based on the result of this experiment, application of 150 kg P ha-1 and Pseudomonas putida strain M5 inoculation can be used to obtain highest forage yield and quality of berseem clover as second crop in the experimental site.  

کلیدواژه‌ها [English]

  • Crude fiber
  • Phosphatase enzyme
  • phosphorus
  • Protein
  • Soil pH
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