پروفیل پروتئینی گندم تحت تنش خشکی و نانوکلات پتاسیم

نوع مقاله: علمی پژوهشی

نویسندگان

1 عضو هیئت علمی دانشگاه

2 دانش‌آموخته بیوتکنولوژی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل ، ایران.

3 استاد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.

4 دانشجوی دکتری فیزیولوژی گیاهان زراعی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران.

چکیده

سیستم­های مقاومتی گیاهان از جمله گندم در مقابل ­تنش­ها توسط روش­های متعددی از جمله مواد شیمیایی مانند نانوکلات پتاسیم تحریک می­گردد. به­منظور بررسی پروفیل پروتئینی گندم تحت تنش خشکی، آزمایشی به صورت فاکتوریل در قالب طرح کاملاً تصادفی با 3 تکرار انجام گرفت. فاکتور اول شامل تنش خشکی در سه سطح (85، 60 و 35 درصد ظرفیت زراعی) و فاکتور دوم سه رقم گندم (زاگرس، چمران و کوهدشت) و فاکتور سوم سه سطح نانوکلات پتاسیم با غلظت­های (صفر، 25، 45 و 65 ppm) بودند. تنش کم­آبی در مرحله­ی سه برگچه­ای بر اساس ظرفیت زراعی به گلدان­ها اعمال و سه روز پس از آن، نانوکلات پتاسیم با غلظت­های مشخص روی برگ­های گندم محلول­پاشی شدند. بررسی­ها نشان دادند در اثر تیمار نانوکلات پتاسیم غلظت اسید آمینه پرولین و کربوهیدرات که در مکانیسم­های دفاعی گیاه نقش اساسی دارند، افزایش یافتند. میزان فعالیت کاتالاز و پلی­فنل­اکسیداز با افزایش غلظت نانوکلات پتاسیم کاهش و برعکس میزان فعالیت پراکسیداز افزایش یافت. نتایج حاصل از مطالعات پروتئومیکسی با استفاده از الکتروفورز دو بعدی نشان داد که بروز پروتئین­های مؤثر که ناشی از نانوکلات پتاسیم در ارتباط مستقیم با سیستم دفاعی به صورت لکه­های 11، 6، 5، 14 و 19 ظاهر شدند. پروتئین­های بیان شده در این آزمایش شامل برخی از آنزیم­های سیستم دفاعی مانند آسکوربات پراکسیدازها، گلوتاتیون s ترانسفرازها و پروتئین­های شوک حرارتی بودند. همچنین، آنزیم­های کلیدی چرخه گلیکولیز و چرخه تری کربوکسیلیک اسید شامل ایزوسیترات دهیدروژناز، تریوزفسفات ایزومراز، فسفوگلیکونات دهیدروژناز، گلیسرآلدهید 3 فسفات دهیدروژناز، فروکتوز بیس فسفات بیان شدند این امر نشانگر این موضــوع می­باشد که تیمار نانوکلات پتاسیم، سطح گلوکز، فروکتـوز و سـاکارز و فـراوانی سـایر آنزیم­های مرتبط با تنش­های زیستی و غیر زیستی را افزایش می­دهد.

کلیدواژه‌ها


عنوان مقاله [English]

Protein Profile in Wheat as Affected by Drought Stress and Nano-Chelate Potassium

نویسندگان [English]

  • sodabeh jahanbakhsh 1
  • Nafiseh Asghari Asghari 2
  • Ali Ebadi 3
  • Nasibeh Tavakoli 4
1 mohaghegh university
2 M.Sc. Graduated of Agricultural Biotechnology. Faculty of Agriculture and Natural Resource, University of Mohaghegh Ardabili, Ardabil, Iran.
3 Prof. Department of Agronomy and Plant Breading, Faculty of Agriculture and Natural Resource, University of Mohaghegh ardabili, Ardabil, Iran.
4 Ph.D. Student of Crop Physiology. Department of Agronomy and Plant Breading, Faculty of Agriculture and Natural Rresource, University of Mohaghegh Ardabili, Ardabil, Iran.
چکیده [English]

Tolerant systems in plants including wheat are affected by several chemical factors, like nano-chelate potassium. Use of nano-chelated potassium under drought stress reduces its negative effects and increase yield. To study protein profiles of wheat under drought stress, a factorial experiment based on completely randomized design with three replications was performed. The first factore was three levels of irrigation (85%, 60% and 35% field capacity), the second factor consisted of three wheat cultivars (Zagros, Chamran and Kuhdasht) and the third factor consisted of four nano-chelated potassium concentrations (zero, 25, 45، 65 ppm). The results showed that concentrations of proline and carbohydrates which play a major role in plant defense mechanisms due to nano-chelated potassium treatment were increased. Catalase and butpolyphenol oxidase activity decreased with increasing concentrations of nano-chelate potassium, while peroxidase activity increased. The results of two dimentional electrophoretic studies showed changes of protein expression, due to the effect of nano-chelated potassium as a direct contact with the defensive system against drought stresses, such as 11, 6, 5, 19, 14 bands. Nano-chelated potassium is also associated with proteins involved in the metabolism of carbohydrates and protein and final energy production.

کلیدواژه‌ها [English]

  • amino acid
  • Drought stress
  • Nano-chelated potassium
  • Proteomics
  • Wheat
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