اثر سطوح مختلف نیتروژن بر خاصیت ضد‌میکروبی و برخی ویژگی‌های فیزیولوژیکی پیاز (Allium cepa L.)

نوع مقاله: علمی پژوهشی

نویسنده

استادیار، دانشکده کشاورزی سنقر، دانشگاه رازی، کرمانشاه، ایران

چکیده

پیاز از جمله گیاهان دارویی است که در طب قدیم کاربرد فراوانی داشته و خاصیت ضدمیکروبی آن اثبات شده است. همچنین در میان سبزی­ها دومین رتبه را بعد از گوجه­فرنگی از نظر ارزش اقتصادی به خود اختصاص داده است پیاز در رژیم غذایی ایرانی­ها اهمیت زیادی دارد. در این مطالعه، اثر سطوح مختلف نیتروژن (56، 112، 168 و 224 میلی­گرم بر لیتر نیتروژن) در محلول غذایی هوگلند بر برخی ویژگی­های فیزیولوژیکی و خاصیت ضد­میکروبی عصاره­ی متانولی سوخ در قالب طرح کاملاً تصادفی و با 3 تکرار مورد بررسی قرار گرفت و بیشترین میزان اثر بازدارندگی عصاره­ی متانولی سوخ در غلظت 30/0 میلی­گرم بر لیتر مشاهده گردید. با توجه به نتایج به­دست آمده نیتروژن تأثیر مثبتی بر میزان خاصیت ضد­میکروبی عصاره­ی سوخ داشت و با افزایش غلظت نیتروژن در محلول غذایی تا غلظت 224 میلی­گرم بر لیتر خاصیت ضد­میکروبی عصاره­ی سوخ افزایش پیدا کرد. باکتری باسیلوس سابتیلیس بیشترین میزان رشد و باکتری استافیلوکوکوس اورئوس کمترین میزان رشد را داشتند. با افزایش غلظت نیتروژن در محلول غذایی میزان فعالیت آنتی­اکسیدانی، فنل کل، فلاونوئید کل و اسید آسکوربیک افزایش نشان داد. افزایش میزان این ترکیبات با افزایش میزان خاصیت آنتی باکتریایی عصاره­ی سوخ همراه بود که بیانگر نقش این ترکیبات در خاصیت آنتی باکتریایی پیاز است. در سال­های اخیر مصرف پیاز به­دلیل طعم و فوایدی که در سلامتی بشر دارد افزایش یافته است. ویژگی­های مفید پیــاز عمـدتاً به میـزان بالای ترکیبات سـولفوره و فلاونـوئیدهای آن مرتبط است، زیرا که نقش آنتی­اکسیدان، ضد­میکروبی و ضد سرطانی این ترکیبات اثبات شده است. نیتروژن بر خاصیت بازدارندگی عصاره­ی متانولی سوخ تأثیرگذار بود که این می­تواند به­دلیل تأثیر نیتروژن بر میزان متابولیسم ترکیبات گوگردی و فلاونوئیدهای موجود در سوخ باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Nitrogenous Fertilizers on Antimicrobial Activity and some Physiological Characteristics of Onion (Allium cepa L.)

نویسنده [English]

  • Masoomeh Amerian
Assistant Professor, Sonqor Agriculture Faculty, Razi University, Kermanshaeh, Iran
چکیده [English]

Onion is a medicinal plant which have many uses in traditional medicine. It is a valuable crop since ancient times and it ranks second after tomato among cultivated vegetables in the world. Also, onion has great importance in the diet of Iranian people. In this study effect of different nitrogen levels (56, 112, 168 and 224 mg.L-1) on antimicrobial activity and some physiological characteristics of bulb extracts were studied.The highest and lowest amounts of inhibitory efficacy of methanol extract of onion bulbs at concentration of 0.30 mg.L-1 against Pseudomonas aeruginosa and Bacillus subtilis, were observed. According to the results, different levels of nitrogen had a positive effect on the antimicrobial properties onion bulb extract. With increased nitrogen concentration in nutrient solution, antimicrobial activity onion bulb extracts increased. With the increase in nitrogen concentration in nutrient solution content antioxidant activity, total phenol, total flavonoids, and ascorbic acid were increased. Increasing the concentration of antioxidant compounds was associated with increased antimicrobial activity of onion bulb extract. In recent years, the consumption of onion has increased due to its flavor and health benefits. The beneficial properties of onion are mainly related to its high content of sulfur compounds and flavonoids, and the role of these compounds as antioxidants, anti-microbial and anti-cancer have been proven. Nitrogen was effective on the inhibitory effect of bulb methanol extract, which could be due to the effect of nitrogen on the metabolism of sulfur compounds and flavonoids in bulb.

کلیدواژه‌ها [English]

  • Alcoholic extract
  • Antioxidant Activity
  • Bulb
  • Total flavonoids
  • Total phenol
  • ·
    • · Abdissa, Y., T. Tekalign, and L.M. Pant. 2011. Growth, bulb yield and quality of onion (Allium cepa L.) as influenced by nitrogen and phosphorus fertilization on vertisol I. growth attributes, biomass production and bulb yield. African Journal of Agricultural Research. 6(14): 3252-3258.
    • · Abdou, I.A., M.R. Abou-Zeid, E.L. Sherbeeny, and Z.H. Abou-El-Gheat. 1972. Antimicrobial activities of Allium sativum, Allium cepa, Rephanus sativus, Capsicum frutescens, Eruca sativa, Allium kurrat on bacteria. Qualitas Plantarum Materiae Vegetabiles. 1: 29-35.
    • · Amerian, M. 2016. Study of antimicrobial effect of onion (Allium cepa L.) bulb extract. 2nd National Conference on Herbs and Herbal Medicine. June 02, Tehran.
    • · Antoòenko, K., V. Kreicbergs, M. Dûma, S. Ozola, and M. Dûma. 2010. The qualitative evaluation of grain fortified with selenium. Doctoral Thesis, Jelgava: LLU, pp. 50–95.
    • · Anvari, M., S. Mohebi, and A. Rafie Zade. 2004. Practical methods in microbiology laboratory. Islamic Azad University Publishing. Page 585. (In Persian).
    • · Atanasova, E. 2008. Effect of nitrogen sources on the nitrogenous forms and accumulation of amino acid in head cabbage. Plant, Soil and Environment. 54: 66-71.
    • · Biesiada, A., and A. Kus. 2010. The effect of nitrogen fertilization and irrigation on yielding and nutrition status of sweet basil(Ocimum basilicum L.). Acta Scientiarum Polonorum Horticulture. 9(2): 3-12.
    • · Bolandnazar, S., M. Mollavali, and S.J. Tabatabaei. 2012. Influence of NH4NO3 and K2SO4 on qualitative characteristics of onion. Scientia Horticulturae. 136: 24–28.
    • · Bor, J., H.Y. Chen, and G.C. Yen. 2006. Evaluation of antioxidant activity and inhibitory effect on nitric oxide production of some common vegetables .Journal of Agricultural and Food Chemistry. 54: 1680-1686.
    • · D’Abrosca, B., S. Pacifico, G. Cefarelli, C. Mastellone, and A. Fiorentino. 2007. Limoncella apple, an Italian apple cultivar: phenolic and flavonoid contents and antioxidant activity. Food Chemistry. 104: 1333-1337.
    • · Elhassaneen, Y.A., and M.I. Sanad. 2009. Phenolics, selenium, vitamin C, amino acids and pungency levels and antioxidant activities of two Egyptian onion varieties. American Journal of Food Technology. 8: 1557-4571.
    • · Haile, A., B. Tesfaye, and W. Worku. 2017. Seed yield of onion (Allium cepa L.) as affected by bulb size and inter-row spacing. African Journal of Agricultural Research. 12(12): 987-996.
    • · Johnson, C.D., and D.R. Decoteau. 1996. Nitrogen and potassium fertility affects jalapeno pepper plant growth, pod yield, and pungency. Horticultureal Science. 31: 1119–1123.
    • · Kapur, A., A. Hasković, A. Čopra-Janićijević, L. Klepo, A. Topčagić, I. Tahirović, and E. Sofić. 2012. Spectrophotometric analysis of total ascorbic acid contetnt in various fruits and vegetables. Bulletin of the Chemists and Technologists of Bosnia and Herzegovina. 40-43.
    • · Kyung, K.H., and Y.C. Lee. 2001. Antimicrobial activities of sulfur compounds derived from salk enyl-L-cysteine sulfoxides in allium and brassica. Food Reviews International. 17(2): 183-198.
    • · Kyung, K.H. 2012. Antimicrobial properties of Allium species. Food Biotechnology. 23: 142–147.
    • · Leja, M., G. Wyz golik, and A. Mareczek. 2005. Phenolic compounds of red cabbage as related to different forms of nutritive nitrogen. Horticulture Vegetable Growth. 24(3): 421–428.
    • · Letchamo, W. 1992. A comparative study of chamomile yield, essential oil and flavenoids content under two sowing seasons and nitrogen levels. Acta Horticulture. 306: 375-384.
    • · Lošák, T., J. Hlušek, R. Filipčík, L. Pospíšilová, J. Maňásek, K. Prokeš, F. Buňka, S. Kráčmar, A. Martensson, and F. Orosz. 2010. Effect of nitrogen fertilization on metabolisms of essential and non-essential amino acids in field-grown grain maize (Zea mays L.). Plant, Soil, Environment. 56(12): 574–579.
    • · Mangena, T.O., and N.Y.O. Muyima. 1999. Comparative evaluation of the antimicrobial activities of essential oils of Artemisia after, Pteronia incana and Rosmarinus on selected bacteria and yeast strains. Letters in Applied Microbiology. 28(4): 291-296.
    • · Masaudi, S.B., and M.O. AlBureikan. 2012. Antimicrobial activity of onion juice (Allium cepa L.), honey, and onion-honey mixture on some sensitive and multi-resistant microorganisms. Life Science Journal. 9(2): 125-129.
    • · Nuutila, A.M., R. Puupponen-Pimia, and M. Aarni. 2003. Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity. Food Chemistry. 81: 485–493.
    • · Olga, B., V. Eija, and F. Kurt. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany. 91: 179-194.
    • · Olsen, K.M., R. Slimesad, U.S. Lea, C. Brede, T. Lqvdal, P. Ruoff, M .Verheul, and C. Lillo. 2009. Temperature and nitrogen effects on regulators and products of the flavonoid pathway: experimental and kinetic model studies. Plant, Cell and Environment. 32: 286–299.
    • · Perner, H., S. Rohn, G. Driemel, N. Batt, D. Schwarz, L.W. Kroh, and D. Schwarz. 2008. Effect of nitrogen species supply and mycorrhizal colonization on organosulfur and phenolic compounds in onions. Journal of Agricultural and Food Chemistry. 56: 3538–3545.
    • · Prakash, D., B.N. Singh, and G. Upadhyay. 2007. Antioxidant and free radical scavenging activities of phenols from onion (Allium cepaL.). Food Chemistry. 102: 1389–1393.
    • · Ramos, F.A., Y. Takaishi, M.S.Y. Shirotori Kawaguchi, K. Tsuchiy, H. Shibata, T. Higuti, T. Tadokoro, and M. Takeuchi. 2006. Antibacterial and antioxidant activities of 36-Quercetin oxidation products from yellow onion (Allium cepa L.) skin. Journal of Agricultural and Food Chemistry. 54: 3551-3557.
    • · Randle, W.M. 2000. Increasing nitrogen concentration in hydroponic solutions affects onion flavor and bulb quality. Journal of the American Society Horticultural Science. 125(2): 254–259.
    • · Ruth, G.A., E. Neval, and S.H. Lenwood. 2004.Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany. 372: 1331-1341.
    • · Sady, W., S. Rozek, M. Leja, and A. Mareczek. 1999. Spring cabbage yield and quality as related to nitrogen fertilizer type and method of fertilizer application. Acta Horticulture. 506: 77–80.
    • · Salim Pour, F., A. Mazouji, S.F. Mozafar, and G. Barzin. 2014. Compare antibacterial properties of essential oil in four species of medicinal plants Salvia L. Journal of MedicalResearch. 37(4): 205-210. (In Persian)
    • · Santas, J., R. Carbo, M.H. Gordon, and M.P. Almajano. 2008.Comparison of the antioxidant activity of two Spanish onion varieties. Food Chemistry. 107: 1210–1216.
    • · Singleton, V.L., and J.A. Rossi. 1965. Colorimetry of total phenolic with phosphomolydic phosphotungstic acid reagents. American Journal of Entomology Viticulture. 16: 144-158.
    • · Smolen, S., and W. Sady. 2009. The effect of various nitrogen fertilization and foliar nutrition regimes on the concentrations of sugars, carotenoids and phenolic compounds in carrot (Daucus carota L.). Scientia Horticulturae. 120: 315–324.
    • · Stewart, A.J., W. Chapman, G. Jenkin, I. Graham, T. Martin, and A. Crozier. 2001. The effect of nitrogen and phosphorus deficiency on flavonol accumulation in plant tissues. Plant, Cell and Environment. 24:1189–1197.
    • · Wilson, E.A., and B. Demmig-Adams. 2007. Antioxidant, anti-inflammatory, and antimicrobial properties of garlic and onions. Nutrition and Food Science. 37(3): 178–183.

    · Benkeblia, N. 2004. Antimicrobial activityof essential oil extracts of various onions (Allium cepa L.) and garlic (Allium sativum L.). Lebensmittel-Wissenschaft und-Technologie. 37: 263–268.

    · Coolong, T.W., and W.M. Randle .2003. Ammonium nitrate fertility levels influence flavor development in hydroponically grown ‘Granex 33’ onion. Journal of the Science of Food and Agriculture. 83: 477–482.

    · Huang, Y., J. Xu, and Q. Hu. 2005. Effect of selenium on preservation quality of green tea during autumn tea-processing season. Journal of Agricultural and Food Chemistry. 53(19):744-747.

    · Kim, J., M.R. Marshall, and C. Wei. 1995. Antibacterial activity of some essential oil components against five food borne pathogens. Journal of Agricultural and Food Chemistry. 43: 2839–2845.

    · Wook Kim, J., J.E. Huh, S.H. Kyung, and K.H. Kyu Hang Kyung. 2004. Antimicrobial activity of Alk enyl Sulfides found in essential oils of garlic and onion. Food Science and Biotechnology. 13(2): 235–239.

    Yang, J., K.J. Meyers, J.V.D. Heide, and R.H. Liu. 2004. Varietal differences in phenolic content and antioxidant and anti-proliferative activities of onions. Journal of Agricultural and Food Chemistry. 52: 6787-6793.