تغییرات عملکرد و اجزای روغن دانه خردل سیاه (Brassica nigra L.) بر اثر باکتری ها و تنظیم کننده های رشد در شرایط تنش کادمیوم

نوع مقاله: علمی پژوهشی


1 دانشجوی دکتری اکولوژی گیاهان زراعی، دانشگاه محقق اردبیلی، اردبیل، ایران

2 دانشیار گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

3 استادیار گروه خاک‌شناسی، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران

4 استاد گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران.


کادمیوم یکی از آلاینده­های اولیه خاک از نوع فلزات سنگین می­باشد و گیاهان تیره شب­بو با تولید وزن خشک بالا قادر به تجمع مقادیر بسیار بالایی از فلزات سنگین از جمله کادمیوم می­باشند. به­منظور ارزیابی تغییرات اسیدهای چرب، درصد روغن دانه و عملکرد و اجزای عملکرد خردل سیاه تحت تاثیر باکتری­های محرک و تنظیم کننده­های رشد گیاه در شرایط تنش کادمیوم آزمایشی به­صورت فاکتوریل در قالب طرح بلوک­های کامل تصادفی در گلخانه تحقیقاتی دانشگاه محقق اردبیلی با چهار تکرار اجرا شد. تیمارهای آزمایشی شامل دو سطح کادمیوم (صفر و 100 میلی­گرم بر کیلوگرم خاک)، سه سطح باکتری (شاهد، آزوسپریلوم و سودوموناس) و سه سطح تنظیم کننده­های رشد (شاهد، سالیسیلیک اسید و براسینواستروئید) بودند. مقایسات میانگین­ها نشان داد که کادمیوم موجب کاهش معنی­دار تعداد نیام در بوته، تعداد دانه در نیام، وزن خشک ریشه، درصد روغن دانه، اولئیک اسید، لینولئیک اسید، ایکوزنوئیک اسید و اروسیک اسید گردید. باکتری­های محرک رشد منجر به افزایش معنی­دار مقادیر صفات مذکور و کاهش معنی­دار پالمتیک اسید شدند. محلول­پاشی با سالیسیلیک اسید و براسینواستروئید به افزایش معنی­دار محصول تک بوته، وزن خشک اندام هوایی و ریشه، درصد روغن دانه و تمام انواع اسیدهای چرب غیراشباع انجامید، در حالی­که استئاریک اسید و اسیدهای چرب اشباع را کاهش داد. اثر متقابل کادمیوم × باکتری نشان داد که در هر دو سطح کادمیوم، باکتری­ها موجب افزایش معنی­دار محصول تک بوته، وزن هزار دانه، وزن خشک اندام هوایی، لینولنیک اسید و اسیدهای چرب غیراشباع و کاهش معنی­دار استئاریک و اسیدهای چرب اشباع گردیدند در حالی­که کادمیوم نتیجه­ای برعکس باکتری­ها در این صفات نشان داد. در اثر متقابل کادمیوم × هورمون هم تیمار هورمونی در هر دو سطح کادمیوم منجر به کاهش معنی­دار پالمیتیک و افزایش اسیدهای چرب غیراشباع شد و کاربرد کادمیوم نیز نتیجه عکس داشت.


عنوان مقاله [English]

Changes in Seed Oil Yield and its Components of Black Mustard (Brassica nigra L.) as Affected by Rhizobacteria and Growth Regulators under Cadmium Stress Conditions

نویسندگان [English]

  • Ali Barghi 1
  • Abdolghayoum Gholipoori 2
  • Akbar Ghavidel 3
  • Mohammad Sedghi 4
1 Ph.D. Student of Agro-Ecology, University of Mohaghegh Ardabili, Ardabil, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
3 Assistant Professor, Department of Soil Science, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
4 Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]

Cadmium is one of the soil primary pollutants which is categorized as heavy metals and brassicaceae family are able to accumulate high amounts of heavy metals such as cadmium by producing high amounts of dry matter. In order to evaluate fatty acids variation, seed oil percentage, yield and yield components of black mustard as affected by growth promoting rhizobacteria and growth regulators under cadmium stress condition, a factorial experiment based on randomized complete block design was conducted at the Research Green House of Mohaghegh Ardabili University with four replications. Experimental treatments were two cadmium levels (0 and 100 milligrams per kilograms of soil), three rhizobacteria levels (control, Azospirillum and Pseudomonas) and three growth regulator levels (control, salicylic acid and brassinosteroid). Mean comparisons indicated that cadmium treatment decreased pods number per plant, grain number per pod, root dry weight, seed oil percentage, oleic, linoleic, eicosenoic and erucic acid percentages, significantly. Growth promoting rhizobacteria increased all of above mentioned parameters significantly, where as, it decreased palmitic acid content. Plant growth regulating sprays resulted in a significant increment in plant yield, shoot and root dry weight, seed oil percentage and all kinds of unsaturated fatty acids while it reduced stearic acid content and saturated fatty acids. The interaction of cadmium×rhizobacteria indicated that both cadmium levels used, along with rhizobacteria treatment, increased plant yield, thousand seed weight, shoot dry weight, linolenic acid and unsaturated fatty acids, where as decreased stearic and saturated fatty acids, significantly. In general, the effects of cadmium on these traits were different. Interaction of cadmium×growth regulators, spray application of growth regulators, under both cadmium levels, reduced palmitic acid and induced unsaturated fatty acids significantly and cadmium application had an inverse result.

کلیدواژه‌ها [English]

  • Cadmium
  • fatty acid
  • Mustard
  • Plant growth regulator
  • Rhizobacteria

· Ahmad, P., M. Sarwat, N.A. Bhat, M.R. Wani, A.G. Kazi, and L.S. Tran. 2015. Alleviation of cadmium toxicity in Brassica juncea L. (Czern. & Coss.) by calcium application involves various physiological and biochemical strategies. Plos One. 10(1): e0114571. doi:10.1371/journal.pone.0114571.
· Aksouh, N.M., B.C. Jacobs, F.L. Stoddart, and R.J. Mailer. 2001. Respone of canola to different heat stresses. Australian Journal of Agricultural Researches. 52: 817-824.
· Ali, H., E. EKhan, and M.A. Sajad. 2013. Phytoremediation of heavy metals concepts and applications. Chemosphere. 91: 869-881.
· Anonymus. 1993. Official methods and recommended practices. The American Oil Chemists Society, Champaign.
· Anonymus. 2002. Official methods of analysis of AOAC international. 17th Ed. MD, USA.
· Bell, J., P. Tanhuanpää, R. Kalendar, A.H. Schulman, and E. Kiviharju. 2007. A major gene for grain cadmium accumulation in oat (Avena sativa L.). Genome. 50: 588-594.
· Ben Ammar, W., I. Nouairi, M. Zarrouk, and F. Jemal. 2008. The effect of cadmium on lipid and fatty acid biosynthesis in tomato leaves. Biologia. 63(1): 86-93.
· Ben Youssef, N., I. Nouairi, S. Ben Temime, W. Taamalli, M. Zarrouk, M.H. Ghorbal, and D. Ben Miled Daoud. 2005. Effets du cadmium sur le metabolisme des lipides de plantules de colza (Brassica napus L.). Biologia. 328: 745–757.
· Broadley, M., M.J. Willey, J.C. Wilkins, A.J.M. Baker, A. Mead, and P.J. White. 2001. Phylogenetic variation in heavy metal accumulation in angiosperms. New Phytologist. 152: 9-27.
· Choudhury, A., and I.R. Kennedy. 2004. Prospects and potentials for systems of biological nitrogen fixation in sustainable rice production. Biology and Fertility of Soils. 39: 219-227.
· Dhanasekar, R., and R. Dhandapani. 2012. Effect of biofertilizers on the growth of Helianthus annuus. International Journal of Plant, Animal and Environmental Sciences. 2: 143-147.
· Feng, J., Q. Shi, X. Wang, M. Wei, F. Yang, and H. Xu. 2010. Silicon supplementation ameliorated the inhibition of photosynthesis and nitrate metabolism by cadmium (Cd) toxicity in Cucumis sativus L. Scientia Horticulture. 123: 521–530.
· Glick, B.R. 2010. Using soil bacteria to facilitate phytoremediation. Biotechnological Advances. 28: 367-374.
· Grusak, M.A., and D. Dellapenna. 1999.Improving the nutrient composition of plants to enhance human nutrition and health. Annual Revolution of Plant Physiology and Plant Molecular Biology. 50: 133-161.
· Heshmati, S., M. Amini Dehaghi, and K. Fathi Amirkhiz. 2016. Effects of biological and chemical phosphorous fertilizer on grain yield, oil seed and fatty acids of spring safflower in water deficit conditions. Iranian Journal of Field Crop Science. 48: 159-169. (In Persian).
· Horvath, E., G. Szalai, and T. Janda. 2007. Induction of abiotic stress tolerance by salicylic acid signaling. Journal of Plant Growth Regulation. 26: 290–300.
· Hunt, R.W., S. Chinnasamy, A. Bhatnagar, and K.C. Das. 2010. Effect of biochemical stimulants on biomass productivity and metabolite content of the microalga, Chlorella sorokiniana. Applications in Biochemistery and Biotechnology. 162(8): 2400–2414.
· Ildiko, S.G., K.A. Klara, T.M. Marianna, B.A. gnes, M.B. Zsuzsanna, and C. Balint. 2006. The effect of radio frequency heat treatment on nutritional and colloid-chemical properties of different white mustard (Sinapis alba L.) varieties. Innovative Food Science and Emerging Technology. 7: 74–79.
· Jeong, S., H.S. Moon, K. Nam, J.Y. Kim, and T.S. Kim. 2012. Application of phosphate-solubilizing bacteria for enhancing bioavailability and phytoextraction of cadmium (Cd) from polluted soil. Chemosphere. 88: 204-210.
· Khalid, S., M. Shahid, N.K. Niazi, B. Murtaza, and I. Bibi. 2017. A comparison of technologies for remediation of heavy metal contaminated soils. Journal of Geochemistery Exploration. 182: 247–268.
· Khosravi, A., R. Seyedsharifi, and A.A. Imani. 2014. Study of seed inoculation with azotobacter and psedomunas and nitrogen application timing on yield, fertilizer use efficiency and grain filling rate of sunflower. Journal of Crops Improvement. 16: 139-155.
· Kraiser, T., D. Gras, A.G. Gutièrrez, B. Gonzalez, and A.R. Gutièrrez. 2011. A holistic view of nitrogen acquisition in plants. Journal of Experimental Botany. 62: 1455-1466.
· Lebeau, T., A. Braud, and K. Jézéquel. 2008. Performance of bioaugmentation-assisted phytoextraction applied to metal contaminated soils: a review. Environmental Pollution. 153: 497-522.
· Liu, J., B.H. Liao, H. Zhou, Y. Zhang, M. Zeng, Y.X. Huang, and Q.R. Zeng. 2010. Main characteristics of physiological-ecological dynamics of soybean during the growth cycle under Cd stress. Acta Ecologia Sinica. 30: 333–340.
· Lu, H., Z. Li, J. Wu, Y. Shen, Y. Li, B. Zou, Y. Tang, and P. Zhuang. 2017. Influences of calcium silicate on chemical forms and subcellular distribution of cadmium in Amaranthus hypochondriacus L. Scientific Reports. 7: 45-83.
· Ma, Y., M. Rajkumar, and H. Freitas. 2009. Isolation and characterization of Ni mobilizing PGPB from serpentine soils and their potential in promoting plant growth and Ni accumulation by Brassica spp. Chemosphere. 75: 719-725.
· Naghizadeh, M., and R. Kabiri. 2017. Effect of foliar application of salicylic acid on some of physiological characteristics of corn (Zea mays L.) under drought stress conditions. Environmental Stresses in Crop Sciences. 9: 315-327. (In Persian).
· Nouairi, I., T. Ghnaya, N. Ben Youssef, M. Zarrouk, and M. Habib Ghorbel. 2006. Changes in content and fatty acid profiles of total lipids of two halophytes: Sesuvium portulacastrum and Mesembryanthemum crystallinum under cadmium stress. Journal of Plant Physiology. 163: 1198–1202.
· Rady, M.M., and G.F. Mohamed. 2015. Modulation of salt stress effects on the growth, physio-chemical attributes and yields of Phaseolus vulgaris L. plants by the combined application of salicylic acid and Moringa oleifera leaf extract. Scientia Horticulture. 193: 105–113.
· Rajkumar, M., N. Ae, and H. Freitas. 2009. Endophytic bacteria and their potential to enhance heavy metal phytoextraction. Chemosphere. 77: 153-160.
· Rajkumar, M., S. Sandhya, M.N.V. Prasad, and H. Freitas. 2012. Perspectives of plant associated microbes in heavy metal phytoremediation. Biotechnological Advances. 30: 1562-1574.
· Sawan, Z.M., S.A. Hafez, A.E. Basyony, and A.R. Alkassas. 2007. Nitrogen, potassium and plant growth retardant effects on oil content and quality of cotton seed. Grasasy Aceites. 58(3): 243-251.
· Shakirova, F.M., A.R. Sakhabutdinova, M.V. Bezrukova, R.A. Fatkhutdinova, and D.R. Fatkhutdinova. 2003. Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Science. 164: 317-322.
· Sharma, A., and A. Dhiman. 2014. Nickel and cadmium toxicity in plants. Journal of Pharmaceutical Science Innovation. 2(2): 20-24.
· Silva, L.R., M.J. Pereira, J. Azevedo, R. Mulas, E. Velasquez, F. Gonzalez-Anders, P. Valentao, and P.B. Andrade. 2013. Inoculation with Bradyrhizobium japonicum enhances the organic and fatty acids content of soybean (Glycine max L. Merrill) seeds. Food Chemistry. 141: 3636-3648.
· Tikhonovich, I.A., and N.A. Provorov. 2011. Microbiology is the basis of sustainable agriculture: An opinion. Annuals of Applied Biology. 159: 155-168.
· Upreti, K.K., and M. Sharma. 2016. Role of plant growth regulators in abiotic stress tolerance. Abiotic Stress Physiology in Horticultural Crops. 58: 19-47.
· Vardhini, B.V. 2013. Brassinosteroids, role for amino acids, peptides and amines modulation in stressed plants- A review In: Anjum, N.A., S.S. Gill, and R. Gill (Eds.). Plant adaptation to environmental change: Significance of amino acids and their derivatives. International of Nosworthy Way, Wallingford OX10 8DE, United Kingdom. pp. 300-316.
· Wanasundara Janitha, P.D. 2011. Proteins of brassicaceae oilseeds and their potential as a plant protein source. Critical Reviews in Food Science and Nutrition. 51: 635-677.
· Weyens, N., S. Taghavi, T. Barac, D. Van der Lelie, J. Boulet, T. Artois, R. Carleer, and J. Vangronsveld. 2009. Bacteria associated with oak and ash on a TCE-contaminated site: characterization of isolates with potential to avoid evapotranspiration of TCE. EnvironmentalScience and Pollution Research. 16: 830-843.
· Zhang, S., H. Lin, L. Deng, G. Gong, Y. Jia, X. Xu, T. Li, Y. Li, and H. Chen. 2013. Cadmium tolerance and accumulation characteristics of Siegesbeckia orientalis L. Ecological Engineering. 51: 133-139.
· Zhang, S., M. Chen, T. Li, X. Xu, and L. Deng. 2010. A newly found cadmium accumulator-Malva sinensis Cavan. Journal of Hazardous Materials. 173: 705-709.
. Zhuang, X., J. Chen, and H. Shim. 2007. New advances in plant growth-promoting rhizobacteria for bioremediation. Environment International. 33: 406-413.