اثر هیومیک اسید بر فعالیت آنزیم‎‌‎های آنتی اکسیدانت و عملکرد کرچک ‏‏(‏Ricinus commonis L.‎‏) در شرایط کمبود آب ‏

نوع مقاله : علمی پژوهشی


استادیار گروه زراعت، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران


تاثیر هیومیک اسید بر فعالیت آنزیم های آنتی اکسیدانت در کرچک با اجرای آزمایشی به صورت اسپلیت پلات در قالب طرح بلوک­های کامل تصادفی در چهار تکرار در سال 1394 در مزرعه تحقیقاتی دانشگاه آزاد اسلامی واحد کرج بررسی شد. فاکتورهای آزمایش شامل تیمارهای آبیاری با 3 سطح شامل آبیاری در 80 درصد ظرفیت زراعی (S1) (نرمال)، آبیاری در 65 درصد ظرفیت زراعی (S2) (تنش ملایم) و آبیاری در 50 درصد ظرفیت زراعی (S3) (تنش شدید) در کرت اصلی و هیومیک اسید در 4 سطح (عدم محلول­پاشی، 1، 2 و 3 بار محلول­پاشی) در کرت فرعی در نظر گرفته شدند. در این بررسی عملکرد دانه، وزن صد دانه، تعداد کپسول در بوته و آنزیم­های آنتی اکسیدانت مورد ارزیابی قرار گرفتند. نتایج نشان داد که اثر محلول­پاشی هیومیک اسید بر وزن صد دانه و میزان آنزیم­های آنتی اکسیدانت در سطح 1% معنی­دار بوده و مقایسه میانگین­ها حاکی از این بود که محلول­پاشی هیومیک اسید می­تواند اثر کمبود آب را تا حدودی جبران کند به­طوری­که میزان سوپر اکسید دیسموتاز، گلوتاتیون پراکسیداز و کاتالاز در شرایط سه بار محلول­پاشی به ترتیب در S2،  8/38، 75/34 و 88/37 و در S3، 63/26، 15/41 و 14/43 درصد نسبت به عدم محلول­پاشی با هیومیک اسید افزایش یافتند. در مجموع، می­توان گفت هیومیک اسید به دلیل اثرات مختلف فیزیولوژیکی علاوه بر افزایش عملکرد کرچک، می تواند در جهت کاهش اثرات تنش کمبود آب نقش مؤثری ایفا نماید، به طوری­که افزایش میزان فعالیت آنزیم­های آنتی اکسیدانت تحت تاثیر این ترکیب می­تواند یک راهکار در مناطق خشک به حساب آمده و کاهش عملکرد را تا حدودی جبران کند.


عنوان مقاله [English]

Effect of Humic Acid on Activity of Antioxidant Enzymes and ‎Yield of Castor Bean (Ricinus commonis) under Water Deficit ‎Condition

نویسنده [English]

  • Mohammad Reza Dadnia
Assistant Prof., Derpartment of Agronomy, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran‎
چکیده [English]

To evaluate the effect of humic acid on activity of antioxidant enzymes in castor bean a split plot experiment based on completely randomize block design with four replications was carried out at Islamic Azad University, Karaj Branch in 2014. Treatments were irrigation with three levels [irrigation at 80% of field capacity (S1) (normal), irrigation at 65% of field capacity (S2) (mid stress) and irrigation at 50% of field capacity (S3) (high stress)] assigned to main plots and humic acid with four levels (without foliar application, one time, twice and three times foliar application) to sub plots. In this study traits like seed yield, 100 seed weight and antioxidant enzymes were estimated. The results showed that the effect of humic acid was significant at 1% level on 100 seed weight and activity of antioxidant enzymes. Mean comparisons indicate that foliar application of humic acid could compensate relative effect of water deficit. It was also revealed that the rate of superoxide dismutase, glutathione peroxidase and catalase were increased with three times of humic acid foliar applications by 38.8, 34.75 and 37.88 percent under mid stress drought and 26.63, 41.15 and 43.14 percents under high stress drought as compared to control, respectively. Overall, it can be said that use of humic acid have different physiological effects. Humic acid not only increases seed yield of castor bean it also have a positive role in reducing water deficit effects and increasing activity of antioxidant enzymes.

کلیدواژه‌ها [English]

  • Castor bean
  • Catalase
  • Glutathione peroxidase
  • Humic acid
  • superoxide dismutase
  • ‎Water deficit.‎
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